The proposed relationship of Nicholas C. Powers as the father of Anne Louise Powers and Rose Gertrude Powers is supported by DNA evidence indicating shared ancestry within the family lines.

Specifically, DNA evidence confirms a 2nd cousin, once removed relationship between PSS (a descendant of the Rose Gertrude Powers ancestral line) and MH (a descendant of the Anne Louise Powers ancestral line) with a shared segment of 151.29 cM across 8 HIR segments.

This match is consistent with their placement within these shared ancestral branches.