The maternal relationship between Augusta Hackbarth and her daughter Hetwig Raguse is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant SS.

The segmental (via DNA segment triangulation) and total DNA shared between SS and other confirmed descendants of Wilhelm Carl Hackbarth/Anna Dorothea Hafemann is consistent with the expected ranges for their respective relationships. Since SS descends from Wilhelm Carl Hackbarth/Anna Dorothea Hafemann through Augusta Hackbarth and her daughter Hetwig Raguse, the presence of significant shared DNA between SS and other known descendants of Wilhelm Carl Hackbarth/Anna Dorothea Hafemann validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple Wilhelm Carl Hackbarth/Anna Dorothea Hafemann through Augusta Hackbarth to Hetwig Raguse, and ultimately to SS, confirming this direct ancestral connection through multiple generations.