The paternal relationship between James Watson and his son John Watson is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant MC.

The segmental (via DNA segment triangulation) and total DNA shared between MC and other confirmed descendants of Henry Watson/Sarah is consistent with the expected ranges for their respective relationships. Since MC descends from Henry Watson/Sarah through James Watson and his son John Watson, the presence of significant shared DNA between MC and other known descendants of Henry Watson/Sarah validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple Henry Watson/Sarah through James Watson to John Watson, and ultimately to MC, confirming this direct ancestral connection through multiple generations.