The paternal relationship between James Alford Harvill and his daughter Myrtle Iona Harvill is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant KU.

The segmental (via DNA segment triangulation) and total DNA shared between KU and other confirmed descendants of Matthias Kelly Harvill/Sarah Burkhart is consistent with the expected ranges for their respective relationships. Since KU descends from Matthias Kelly Harvill/Sarah Burkhart through James Alford Harvill and his daughter Myrtle Iona Harvill, the presence of significant shared DNA between KU and other known descendants of Matthias Kelly Harvill/Sarah Burkhart validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple Matthias Kelly Harvill/Sarah Burkhart through James Alford Harvill to Myrtle Iona Harvill, and ultimately to KU, confirming this direct ancestral connection through multiple generations.