The paternal relationship between Joseph Henry Mixer and his daughter Margaret A "Maggie" Mixer is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant TH.

The segmental (via DNA segment triangulation) and total DNA shared between TH and other confirmed descendants of Solomon "Salmon" Mixer/Margaret Hartman is consistent with the expected ranges for their respective relationships. Since TH descends from Solomon "Salmon" Mixer/Margaret Hartman through Joseph Henry Mixer and his daughter Margaret A "Maggie" Mixer, the presence of significant shared DNA between TH and other known descendants of Solomon "Salmon" Mixer/Margaret Hartman validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple Solomon "Salmon" Mixer/Margaret Hartman through Joseph Henry Mixer to Margaret A "Maggie" Mixer, and ultimately to TH, confirming this direct ancestral connection through multiple generations.