The maternal relationship between Cassandra House and her daughter Mary E. Hall is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant TR.

The segmental (via DNA segment triangulation) and total DNA shared between TR and other confirmed descendants of John House is consistent with the expected ranges for their respective relationships. Since TR descends from John House through Cassandra House and her daughter Mary E. Hall, the presence of significant shared DNA between TR and other known descendants of John House validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA John House through Cassandra House to Mary E. Hall, and ultimately to TR, confirming this direct ancestral connection through multiple generations.