The maternal relationship between Lucinda Cynthia House and her son Samuel H. Hafer is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant MM.

The segmental (via DNA segment triangulation) and total DNA shared between MM and other confirmed descendants of John House/Elizabeth Hughes is consistent with the expected ranges for their respective relationships. Since MM descends from John House/Elizabeth Hughes through Lucinda Cynthia House and her son Samuel H. Hafer, the presence of significant shared DNA between MM and other known descendants of John House/Elizabeth Hughes validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple John House/Elizabeth Hughes through Lucinda Cynthia House to Samuel H. Hafer, and ultimately to MM, confirming this direct ancestral connection through multiple generations.