The maternal relationship between Margaret Elizabeth Carner and her son James Roy Moore is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant RD.

The segmental (via DNA segment triangulation) and total DNA shared between RD and other confirmed descendants of Jacob Carner/Sarah Ellen Albertson is consistent with the expected ranges for their respective relationships. Since RD descends from Jacob Carner/Sarah Ellen Albertson through Margaret Elizabeth Carner and her son James Roy Moore, the presence of significant shared DNA between RD and other known descendants of Jacob Carner/Sarah Ellen Albertson validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple Jacob Carner/Sarah Ellen Albertson through Margaret Elizabeth Carner to James Roy Moore, and ultimately to RD, confirming this direct ancestral connection through multiple generations.