The maternal relationship between Martha Jane Pendleton and her son John Pendleton Brashears is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JPS.

The segmental (via DNA segment triangulation) and total DNA shared between JPS and other confirmed descendants of John T Pendleton/Clarissa Colley is consistent with the expected ranges for their respective relationships. Since JPS descends from John T Pendleton/Clarissa Colley through Martha Jane Pendleton and her son John Pendleton Brashears, the presence of significant shared DNA between JPS and other known descendants of John T Pendleton/Clarissa Colley validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple John T Pendleton/Clarissa Colley through Martha Jane Pendleton to John Pendleton Brashears, and ultimately to JPS, confirming this direct ancestral connection through multiple generations.