The paternal relationship between Jacob III Mann and his son
Gholston Mann is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant EM.
The segmental (via DNA segment triangulation) and total DNA shared between EM and other confirmed descendants of
Mathias Sr Kessinger is consistent with the expected ranges for their respective relationships. Since EM descends from Mathias Sr Kessinger through Jacob III Mann and his son Gholston Mann, the presence of significant shared DNA between EM and other known descendants of Mathias Sr Kessinger validates the generational links along this paternal line.
This supports the conclusion that DNA was passed from MRCA Mathias Sr Kessinger through Jacob III Mann to Gholston Mann, and ultimately to EM, confirming this direct ancestral connection through multiple generations.