The maternal relationship between Charlotte Justine Glander and her daughter Bertha Christine Wilhelmine Radke is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant HJS.

The segmental (via DNA segment triangulation) and total DNA shared between HJS and other confirmed descendants of Christian Ludwig Glander/Anna Christina Wojahn is consistent with the expected ranges for their respective relationships. Since HJS descends from Christian Ludwig Glander/Anna Christina Wojahn through Charlotte Justine Glander and her daughter Bertha Christine Wilhelmine Radke, the presence of significant shared DNA between HJS and other known descendants of Christian Ludwig Glander/Anna Christina Wojahn validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple Christian Ludwig Glander/Anna Christina Wojahn through Charlotte Justine Glander to Bertha Christine Wilhelmine Radke, and ultimately to HJS, confirming this direct ancestral connection through multiple generations.