The maternal relationship between Mary Doak and her son Joseph P. Matthews is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant RH.

The segmental (via DNA segment triangulation) and total DNA shared between RH and other confirmed descendants of John Doak/Mary Wilson is consistent with the expected ranges for their respective relationships. Since RH descends from John Doak/Mary Wilson through Mary Doak and her son Joseph P. Matthews, the presence of significant shared DNA between RH and other known descendants of John Doak/Mary Wilson validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple John Doak/Mary Wilson through Mary Doak to Joseph P. Matthews, and ultimately to RH, confirming this direct ancestral connection through multiple generations.