Lottie Alma Mixer (1900 - 1980)

The maternal relationship between Lottie Alma Mixer and her DNA tested daughter CB is confirmed by the direct inheritance path of autosomal DNA.

The segmental (via DNA segment triangulation) and total DNA shared between CB and other confirmed descendants of Margaret Hartman is consistent with the expected ranges for their respective relationships. Since CB descends from Margaret Hartman through Lottie Alma Mixer, the presence of significant shared DNA between CB and other known descendants of Margaret Hartman validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA Margaret Hartman through Lottie Alma Mixer to CB, confirming this direct ancestral connection through multiple generations.