The maternal relationship between Lottie Alma Mixer and her daughter CB is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant CB.

The segmental (via DNA segment triangulation) and total DNA shared between CB and other confirmed descendants of Solomon "Salmon" Mixer/Margaret Hartman is consistent with the expected ranges for their respective relationships. Since CB descends from Solomon "Salmon" Mixer/Margaret Hartman through Lottie Alma Mixer and her daughter CB, the presence of significant shared DNA between CB and other known descendants of Solomon "Salmon" Mixer/Margaret Hartman validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple Solomon "Salmon" Mixer/Margaret Hartman through Lottie Alma Mixer to CB, and ultimately to CB, confirming this direct ancestral connection through multiple generations.