The paternal relationship between Samuel Ralph Boylan and his son SRB is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant SRB.

The segmental (via DNA segment triangulation) and total DNA shared between SRB and other confirmed descendants of William Thomas Fowler/Mary Anne Mattingly is consistent with the expected ranges for their respective relationships. Since SRB descends from William Thomas Fowler/Mary Anne Mattingly through Samuel Ralph Boylan and his son SRB, the presence of significant shared DNA between SRB and other known descendants of William Thomas Fowler/Mary Anne Mattingly validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple William Thomas Fowler/Mary Anne Mattingly through Samuel Ralph Boylan to SRB, and ultimately to SRB, confirming this direct ancestral connection through multiple generations.