The paternal relationship between Nathan Elmer Carpenter and his son Orville Edward Carpenter is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JB.

The segmental (via DNA segment triangulation) and total DNA shared between JB and other confirmed descendants of Edward S. Carpenter/Eleanor 'Ellen' House is consistent with the expected ranges for their respective relationships. Since JB descends from Edward S. Carpenter/Eleanor 'Ellen' House through Nathan Elmer Carpenter and his son Orville Edward Carpenter, the presence of significant shared DNA between JB and other known descendants of Edward S. Carpenter/Eleanor 'Ellen' House validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple Edward S. Carpenter/Eleanor 'Ellen' House through Nathan Elmer Carpenter to Orville Edward Carpenter, and ultimately to JB, confirming this direct ancestral connection through multiple generations.