The maternal relationship between Carrie Theodosa Manary and her daughter ENM is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant LW.

The segmental (via DNA segment triangulation) and total DNA shared between LW and other confirmed descendants of James Calvin Smothers/Minerva Jane Thomas is consistent with the expected ranges for their respective relationships. Since LW descends from James Calvin Smothers/Minerva Jane Thomas through Carrie Theodosa Manary and her daughter ENM, the presence of significant shared DNA between LW and other known descendants of James Calvin Smothers/Minerva Jane Thomas validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple James Calvin Smothers/Minerva Jane Thomas through Carrie Theodosa Manary to ENM, and ultimately to LW, confirming this direct ancestral connection through multiple generations.