The maternal relationship between Elizabeth Ann Couch and her daughter Louetta Millheim is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JW.

The segmental (via DNA segment triangulation) and total DNA shared between JW and other confirmed descendants of John Albertson/Elizabeth Hunter is consistent with the expected ranges for their respective relationships. Since JW descends from John Albertson/Elizabeth Hunter through Elizabeth Ann Couch and her daughter Louetta Millheim, the presence of significant shared DNA between JW and other known descendants of John Albertson/Elizabeth Hunter validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple John Albertson/Elizabeth Hunter through Elizabeth Ann Couch to Louetta Millheim, and ultimately to JW, confirming this direct ancestral connection through multiple generations.