The paternal relationship between Jacob Ezekiel Johnston and his daughter
Betty A. Johnston is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant TS.
The segmental (via DNA segment triangulation) and total DNA shared between TS and other confirmed descendants of
Ralph Higginbotham/
Susannah Watson is consistent with the expected ranges for their respective relationships. Since TS descends from Ralph Higginbotham/Susannah Watson through Jacob Ezekiel Johnston and his daughter Betty A. Johnston, the presence of significant shared DNA between TS and other known descendants of Ralph Higginbotham/Susannah Watson validates the generational links along this paternal line.
This supports the conclusion that DNA was passed from MRCA couple Ralph Higginbotham/Susannah Watson through Jacob Ezekiel Johnston to Betty A. Johnston, and ultimately to TS, confirming this direct ancestral connection through multiple generations.