The maternal relationship between Arlene Margaret Wagner and her daughter WF is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant WF.

The segmental (via DNA segment triangulation) and total DNA shared between WF and other confirmed descendants of Stephen Derr Wagner/Minnie Lera Reed is consistent with the expected ranges for their respective relationships. Since WF descends from Stephen Derr Wagner/Minnie Lera Reed through Arlene Margaret Wagner and her daughter WF, the presence of significant shared DNA between WF and other known descendants of Stephen Derr Wagner/Minnie Lera Reed validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple Stephen Derr Wagner/Minnie Lera Reed through Arlene Margaret Wagner to WF, and ultimately to WF, confirming this direct ancestral connection through multiple generations.