The maternal relationship between Ann Koepke and her daughter EM is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant CR.

The segmental (via DNA segment triangulation) and total DNA shared between CR and other confirmed descendants of August Friedrich Ferdinand PAHLOW/Christine Sophia Mailahn is consistent with the expected ranges for their respective relationships. Since CR descends from August Friedrich Ferdinand PAHLOW/Christine Sophia Mailahn through Ann Koepke and her daughter EM, the presence of significant shared DNA between CR and other known descendants of August Friedrich Ferdinand PAHLOW/Christine Sophia Mailahn validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple August Friedrich Ferdinand PAHLOW/Christine Sophia Mailahn through Ann Koepke to EM, and ultimately to CR, confirming this direct ancestral connection through multiple generations.