The maternal relationship between Frances Vivian Upton and her son Samuel Ralph Boylan is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant SRB.

The segmental (via DNA segment triangulation) and total DNA shared between SRB and other confirmed descendants of William Thomas Fowler/Mary Anne Mattingly is consistent with the expected ranges for their respective relationships. Since SRB descends from William Thomas Fowler/Mary Anne Mattingly through Frances Vivian Upton and her son Samuel Ralph Boylan, the presence of significant shared DNA between SRB and other known descendants of William Thomas Fowler/Mary Anne Mattingly validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple William Thomas Fowler/Mary Anne Mattingly through Frances Vivian Upton to Samuel Ralph Boylan, and ultimately to SRB, confirming this direct ancestral connection through multiple generations.