The proposed relationship of Maria Rosetta 'Mattie' Smothers as the mother of Autumn O'Neal 'Bud' Manary and Carrie Theodosa Manary is supported by DNA evidence indicating shared ancestry within the family lines.

Specifically, DNA evidence confirms a distant cousin relationship between LW (a descendant of the Carrie Theodosa Manary ancestral line) and JO (a descendant of the Autumn O'Neal 'Bud' Manary ancestral line) with a shared segment of 61.90 cM across 3 HIR segments.

This match is consistent with their placement within these shared ancestral branches.