The maternal relationship between Maria Rosetta 'Mattie' Smothers and her son Autumn O'Neal 'Bud' Manary is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant JO.

The segmental (via DNA segment triangulation) and total DNA shared between JO and other confirmed descendants of James Calvin Smothers/Minerva Jane Thomas is consistent with the expected ranges for their respective relationships. Since JO descends from James Calvin Smothers/Minerva Jane Thomas through Maria Rosetta 'Mattie' Smothers and her son Autumn O'Neal 'Bud' Manary, the presence of significant shared DNA between JO and other known descendants of James Calvin Smothers/Minerva Jane Thomas validates the generational links along this maternal line.

This supports the conclusion that DNA was passed from MRCA couple James Calvin Smothers/Minerva Jane Thomas through Maria Rosetta 'Mattie' Smothers to Autumn O'Neal 'Bud' Manary, and ultimately to JO, confirming this direct ancestral connection through multiple generations.