The paternal relationship between William Isaac Wagner and his daughter Arlene Margaret Wagner is confirmed by the direct inheritance path of autosomal DNA down to their DNA tested descendant WF.

The segmental (via DNA segment triangulation) and total DNA shared between WF and other confirmed descendants of Stephen Derr Wagner/Minnie Lera Reed is consistent with the expected ranges for their respective relationships. Since WF descends from Stephen Derr Wagner/Minnie Lera Reed through William Isaac Wagner and his daughter Arlene Margaret Wagner, the presence of significant shared DNA between WF and other known descendants of Stephen Derr Wagner/Minnie Lera Reed validates the generational links along this paternal line.

This supports the conclusion that DNA was passed from MRCA couple Stephen Derr Wagner/Minnie Lera Reed through William Isaac Wagner to Arlene Margaret Wagner, and ultimately to WF, confirming this direct ancestral connection through multiple generations.